new products
P383-T-ALL
T-ALL
P430-PLCG2
Cold-induced urticaria
P406-CMT2
CMT2B/2D/2F

improved products
P244-AIP-MEN1-CDKN1B
Multiple endocrine neoplasia (MEN)
P189-CDKL5
Rett syndrome, atypical
P072-MSH6
Hereditary nonpolyposis colon cancer (HNPCC)
P061-Lissencephaly
Lissencephaly
P069-Human Telomere-4
Broad subtelomeric screening
P015-MECP2
RETT syndrome
P064-MR-1
Mental Retardation; 1p-deletion, Williams, Smith-Magenis, Miller-Dieker, DiGeorge, Prader-Willi, Alagille, Saethre-Chotzen, Sotos
P237-DNAI1
Primary ciliary dyskinesia (PCD)
P313-CREBBP
Rubinstein–Taybi syndrome (RSTS)
P218-LPL
LPL deficiency
P205-SH2D1A-XIAP-ITK
Lymphoproliferative syndrome
P169-Hirschsprung-1
Hirschsprung disease, or Aganglionic Megacolon

General Questions on MLPA
  1. What is MLPA?
  2. What are the advantages of MLPA?
  3. What are the limitations of MLPA?
  4. Which equipment is recommended for MLPA analysis?
  5. Can I use the Qiaxcel system for MLPA?
  6. Can I use MLPA on an ALF express?
  7. Are there MLPA kits available for agarose gels?
  8. Is it possible to use MLPA for testing the methylation status of a specific site?
  9. Is it possible to detect point mutations with MLPA?
  10. What is the difference between DNA, RNA, and Methylation MLPA probes?
  11. Is it possible to use SALSA MLPA probes on DNA from other organisms?
  12. Can I use SALSA MLPA kits in diagnostics?
  13. Do I need to confirm MLPA results with other methods?
  14. Can I have my samples tested at MRC-Holland?
  15. Can I order synthetic probes from MRC-Holland?
  16. What do the different version numbers mean?
  17. Is MLPA a patented technology?
  18. Why does MRC-Holland occasionally change the exon numbering or gene name used in its product materials?
Questions about the MLPA technique
  1. To perform MLPA, is it necessary to get training?
  2. Which quality controls are present in SALSA MLPA kits?
  3. How many and what kind of control samples should be included in MLPA experiments?
  4. What should a no DNA control look like in MLPA?
  5. Does MRC-Holland have positive control samples available?
  6. Is the purity and quality of the sample DNA important?
  7. Is it possible to use DNA extracted from paraffin coupes for MLPA?
  8. How important is the buffer used to dissolve the DNA?
  9. Are SALSA MLPA reagents stable?
  10. What are the unique features of the Ligase-65 enzyme?
  11. Does Ligase-65 use ATP or NAD as a cofactor?
  12. Do mismatches close to the ligation site of probes influence results?
  13. Is it possible to compare results from different experiments?
  14. Is it possible to use 0.5 ml tubes?
  15. Why does the DNA MLPA protocol start with 5 min. incubation at 98 °C?
  16. Can I use smaller amounts of the probemix?
  17. Can I decrease the duration of the hybridisation reaction?
  18. Can I reduce the number of cycles in the PCR reaction?
  19. Which factors influence relative probe signals?
  20. Is the amplification of MLPA probes compatible with amplification techniques other than PCR?
  21. Does a touchdown PCR protocol improve results?
  22. Is it possible to use nested PCR techniques?
  23. Can I get the exact sequences recognised by the SALSA probes?
  24. Is it possible for me to design a synthetic probe for a specific gene and add it to an existing SALSA MLPA probemix?
  25. How can I analyse my MLPA data?
  26. What can be the cause of unexpected results in my MLPA peak patterns?
Ordering & payment information
  1. How can I order from MRC-Holland?
  2. How much are the shipping costs?
  3. Do you have a distributor in my country?
  4. If my country is part of the European Union, do I still have to pay VAT (TVA; MWS; BTW; IVA; MOMS)?
  5. Is it possible to obtain a SALSA MLPA sample kit?
  6. Is it possible to order a part of probemix/just one or more probes?
  7. Can I order a probemix for less than 100 reactions?
  8. Can I buy an extra vial of one of the buffers or the PCR primers from MRC Holland?
  9. Is it possible to order custom-designed probes?
  10. What other products are available at MRC Holland?
  11. Are there any other licenses required for performing an MLPA reaction?
  12. How do I pay?
  13. What are the bank details of MRC-Holland?
  14. When I received my order, all dry ice was gone. Will the MLPA kits be damaged?
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