[./productpagepag.html]
[./indexpag.html]
[./support_pagepag.html]
[./contactpagepag.html]
Copyright © 2005 - MRC-Holland
[./indexpag.html]
Home
-
[./productpagepag.html]
Products
-
[./support_pagepag.html]
Support
-
[./article_pagepag.html]
Articles
-
[./contactpagepag.html]
contact
SALSA MLPA KIT P072 MSH6
Germline defects in the MSH2 and MLH1 genes are the major cause of hereditary nonpolyposis colon cancer (HNPCC). The SALSA MLPA kit P003 MSH2-MLH1 provides a simple method to detect deletions in these two genes (Gille, H. et al, British J. Cancer 87, 892-897) and is widely used as a primary screening tool for HNPCC. Germ-line defects in MSH6 at 2p16 are also know to cause HNPCC. Defects in 4 other 4 genes (PMS2, MLH3, MUTYH, MSH3) might also be involved in HNPCC. SALSA MLPA kit P008 MSH2, PMS2 contains probes for all these genes. As many labs do not analyse the complicated PMS2 gene and prefer only testing MLH1, MSH2 and MSH6, we have prepared this P072 MSH6 kit. Whereas the number of deletions/duplications detected by SALSA MLPA kit P003 MLH1/MSH2 can be 10% of all samples tested, this percentage is much lower for this P072 MSH6 kit. The Leiden website on MSH6 mentions that 8% of the detected MSH6 mutations were deletions of one or more exons (www.lumc.nl/4080/DNA/MSH6.html). This P072-A1 MSH6 probemix contains probes for each of the 10 MSH6 exons. For several exons, more than one probe is present. Three probes for the MUTYH gene have also been included. In addition, one probe is included for MSH2 exon 1, one probe at 2 Kb upstream of MSH2 and two probes for the TACSTD1 gene located 15-25 Kb upstream of MSH2. These probes can be useful to confirm and further characterise deletions that involve exon 1 of MSH2, detected with SALSA MLPA kit P003 MSH2/MLH1. For the same reason three MLH1 exon 1 probes are included. This SALSA MLPA kit is designed to detect deletions/duplications of one or more exons of the MSH6 gene. Heterozygote deletions of probe recognition sequences should give a 35-50% reduced relative peak area of the amplification product of that probe. However, mutations and/or polymorphisms very close to the probe ligation site may also result in a reduced relative peak area. Therefore, apparent deletions detected by a single probe always require confirmation by other methods. Please note that most defects in MSH6 are expected to be small (point) mutations, most of which will not be detected by this MLPA test.
Full mix description (pdf)
Last change in probe mix content: Lot 0708 (April 2008) Current Lot Number.: Lot 0708
IMPORTANT NOTICE: MLPA kits are sold by MRC-Holland for research purposes and to demonstrate the possibilities of the MLPA technique. This kit is not CE/FDA certified for use in diagnostic procedures. Salsa MLPA kits are supplied with all necessary buffers and enzymes. Purchase of the Salsa MLPA test kits includes a limited license to use these products for research purposes. The use of this MLPA kit requires a thermocycler with heated lid and sequence type electrophoresis equipment. Different fluorescent PCR primers are available. The MLPA technique has been first described in Nucleic Acid Research 30, e57 (2002)
References 2006 -- Genomic rearrangements in MSH2, MLH1 or MSH6 are rare in HNPCC patients carrying point mutations. 2005 -- Frequency of hereditary non-polyposis colorectal cancer among Uruguayan patients with colorectal cancer.
[./order_infopag.html]
[./products_prenatal_and_postnatalpag.html]
[./products_hereditary_cancer_researchpag.html]
[./products_various_syndromespag.html]
[./products_tumor_characterisationpag.html]
[./products_mrna_analysispag.html]
[./products_methylation_specificpag.html]
[./products_otherpag.html]
[./products_pharmacogeneticspag.html]
[./mlpapricelistpag.html]
[Web Creator] [LMSOFT]