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SALSA MLPA KIT P300 Reference probemix-2
[http://www.geneclinics.org/profiles/hht/details.html]
The P300-A1 reference probemix contains reference probes and control fragments specific for unique human DNA sequences. This probemix has been developed to add to 'home-made' synthetic MLPA probes. The control fragments and reference probes in this P300 probemix facilitate the data analysis of your synthetic probemix and maximise the number of synthetic probes you can include. Next to the P300, MRC-Holland also offers the P200 reference probemix. While the P200-A1 leaves the entire range from 80-170 nt open for the inclusion of synthetic probes, the reference P300-A1 probemix has reference probes distributed over the whole size range of the probemix. This is especially advantageous for correction of the signal sloping of the probe fragments. A normal amount of signal sloping of the MLPA is always expected, and if this effect is present in both reference and samples runs, normalization will remove this artifact. If one wants to maximise the number of synthetic probes that can be included however, the P200 is recommended. Guidelines on how to design synthetic MLPA probes and the necessary RAW program are available on our website www.mlpa.com. Unfortunately, we cannot offer additional support on probe design. Please read our synthetic probe design protocol very carefully before you start designing. This P300-A1 reference probemix contains 15 reference probes. In addition, the Q-fragments, two D-fragments, one chromosome Y-specific and one chromosome X-specific control probe are included. The Q-fragments give a warning when insufficient amounts of sample DNA are used. The D-fragments give a warning when sample DNA was not completely denatured. SALSA MLPA kit P200 / P300 Reference probemix is available for 660 euro per 100 reactions.
Full mix description (pdf)
IMPORTANT NOTICE: MLPA kits are sold by MRC-Holland for research purposes and to demonstrate the possibilities of the MLPA technique. This kit is not CE/FDA certified for use in diagnostic procedures. Salsa MLPA kits are supplied with all necessary buffers and enzymes. Purchase of the Salsa MLPA test kits includes a limited license to use these products for research purposes. The use of this MLPA kit requires a thermocycler with heated lid and sequence type electrophoresis equipment. Different fluorescent PCR primers are available. The MLPA technique has been first described in Nucleic Acid Research 30, e57 (2002)
References
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Last change in probemix content Current lot number
: lot 0408 (April 2008) : lot 0408
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